Recombinant Human TRIM21/RO52 Protein (XB748011) expressed in Confidential. Purity: >90% by SDS-PAGE.
Highlights
●High Purity — >90% purity verified by SDS-PAGE.
Expression system
Confidential
Accession
P19474
Protein length
Confidential
Applications
As a diagnostic tool for Systemic Lupus Erythematosus (SLE) and Sjögren Syndrome (SS).
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
56.33 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
SS-A, E3 ubiquitin-protein ligase TRIM21, TRIM21, Ro(SS-A), 52 kDa Ro protein, Tripartite motif-containing protein 21, RING finger protein 81, 52 kDa ribonucleoprotein autoantigen Ro/SS-A, RNF81, Sjoegren syndrome type A antigen, RO52, RING-type E3 ubiquitin transferase TRIM21, SSA1
Background
E3 ubiquitin-protein ligase TRIM21 is a ~54 kDa protein. E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling.
1. Wada, K. et al. (2006) Biochemical and biophysical research communications 339, 415-21. PMID: 16297862
2. Wada, K. et al. (2006) Biochemical and biophysical research communications 339, 731-6. PMID: 16316627
3. Wada, K. et al. (2006) Biochemical and biophysical research communications 342, 253-8. PMID: 16472766
4. Sabile, A. et al. (2006) Molecular and cellular biology 26, 5994-6004. PMID: 16880511
5. Takahata, M. et al. (2008) Molecular immunology 45, 2045-54. PMID: 18022694
6. Yamochi, T. et al. (2008) Biochemical and biophysical research communications 370, 195-9. PMID: 18361920
7. Higgs, R. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 181, 1780-6. PMID: 18641315
8. Espinosa, A. et al. (2008) Experimental cell research 314, 3605-13. PMID: 18845142
9. Wada, K. et al. (2009) Journal of biochemistry 146, 821-32. PMID: 19675099
10. Kimura, T. et al. (2015) The Journal of cell biology 210, 973-89. PMID: 26347139
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human TRIM21/RO52 Protein
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