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| 카탈로그 번호 | HB027012 |
|---|---|
| 설명 |
Recombinant Human TRIM27 Protein, N-His (HB027012) expressed in E. coli, spanning Ser315-Pro513. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P14373 |
| 단백질 길이 | Ser315-Pro513 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 24.33 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | RING finger protein 76, Zinc finger protein RFP, RFP, TRIM27, RNF76, RING-type E3 ubiquitin transferase TRIM27, Tripartite motif-containing protein 27, Ret finger protein |
| 배경 | Zinc finger protein RFP (TRIM27) is a ~58 kDa protein. E3 ubiquitin-protein ligase that mediates ubiquitination of various substrates and thereby plays a role in diffent processes including proliferation, innate immunity, apoptosis, immune response or autophagy. Ubiquitinates PIK3C2B and inhibits its activity by mediating the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1, leading to promote endosomal F-actin assembly. Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. 1. Zurek, B. et al. (2012) PloS one 7, e41255. PMID: 22829933 2. Zaman, MM. et al. (2013) Molecular and cellular biology 33, 4971-84. PMID: 24144979 3. Cai, J. et al. (2018) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 32, 5238-5249. PMID: 29688809 4. Garcia-Garcia, J. et al. (2023) The FEBS journal 290, 1096-1116. PMID: 36111389 5. Hao, YH. et al. (2013) Cell 152, 1051-64. PMID: 23452853 6. Zheng, Q. et al. (2015) Cell research 25, 1121-36. PMID: 26358190 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human TRIM27 Protein, N-His
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