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| 카탈로그 번호 | HB028012 |
|---|---|
| 설명 |
Recombinant Human UGT2B7 Protein, N-His (HB028012) expressed in E. coli, spanning Ala285-Asp451. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P16662 |
| 단백질 길이 | Ala285-Asp451 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 20.80 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | UGTB2B9, UDPGTh-2, UDPGT 2B9, UDP-glucuronosyltransferase 2B7, 3, 4-catechol estrogen-specific UDPGT, UGT2B7, UDPGT 2B7, UDP-glucuronosyltransferase 2B9 |
| 배경 | UDP-glucuronosyltransferase 2B7 (UGT2B7) is a ~60 kDa protein. UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile. Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds. Catalyzes the glucuronidation of endogenous steroid hormones such as androgens (epitestosterone, androsterone) and estrogens (estradiol, epiestradiol, estriol, catechol estrogens). Also regulates the levels of retinoic acid, a major metabolite of vitamin A involved in apoptosis, cellular growth and differentiation, and embryonic development. Contributes to bile acid (BA) detoxification by catalyzing the glucuronidation of BA substrates, which are natural detergents for dietary lipids absorption. 1. Samokyszyn, VM. et al. (2000) The Journal of biological chemistry 275, 6908-14. PMID: 10702251 2. Picard, N. et al. (2005) Drug metabolism and disposition: the biological fate of chemicals 33, 139-46. PMID: 15470161 3. Lépine, J. et al. (2004) The Journal of clinical endocrinology and metabolism 89, 5222-32. PMID: 15472229 4. Miley, MJ. et al. (2007) Journal of molecular biology 369, 498-511. PMID: 17442341 5. Alonen, A. et al. (2008) Biochemical pharmacology 76, 763-72. PMID: 18674515 6. Itäaho, K. et al. (2008) Drug metabolism and disposition: the biological fate of chemicals 36, 2307-15. PMID: 18719240 7. Sten, T. et al. (2009) Drug metabolism and disposition: the biological fate of chemicals 37, 417-23. PMID: 19022937 8. Sneitz, N. et al. (2013) Drug metabolism and disposition: the biological fate of chemicals 41, 582-91. PMID: 23288867 9. Perreault, M. et al. (2013) Drug metabolism and disposition: the biological fate of chemicals 41, 1616-20. PMID: 23756265 10. Kallionpää, RA. et al. (2015) The Journal of steroid biochemistry and molecular biology 154, 104-11. PMID: 26220143 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human UGT2B7 Protein, N-His
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