中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HV501022 |
|---|---|
| 설명 |
Recombinant Human USP25 Protein, N-His (HV501022) expressed in E. coli, spanning Glu862-Arg1055. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q9UHP3 |
| 단백질 길이 | Glu862-Arg1055 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 25.44 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Ubiquitin-specific-processing protease 25, Ubiquitin thioesterase 25, USP21, USP on chromosome 21, Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25, USP25 |
| 배경 | Ubiquitin carboxyl-terminal hydrolase 25 (USP25) is a ~122 kDa protein. Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions in various biological processes including inflammation and immune response. Modulates the Wnt/beta-catenin pathway by deubiquitinating and stabilizing tankyrases TNKS1 and TNKS2. Regulates KEAP1-NRF2 axis in the defense against oxidative assaults by deubiquitinating KEAP1 and protecting it from degradation leading to degradation of the NRF2 transcription factor that is responsible for mounting an anti-oxidation gene expression program. Positively regulates RNA virus-induced innate signaling by interacting with and deubiquitinating ERLIN1 and ERLIN2. In turn, restricts virus production by regulating cholesterol biosynthetic flux. 1. Qian, G. et al. (2018) Biochemical and biophysical research communications 498, 537-543. PMID: 29518389 2. Teo, QW. et al. (2023) Developmental cell 58, 2495-2509.e6. PMID: 37683630 3. Xu, D. et al. (2017) Genes & development 31, 1024-1035. PMID: 28619731 4. Sauer, F. et al. (2019) Molecular cell 74, 421-435.e10. PMID: 30926243 5. Fan, CX. et al. (2024) Brain : a journal of neurology 147, 3442-3457. PMID: 38875478 6. Cai, C. et al. (2023) Nature communications 14, 3648. PMID: 37339955 7. Zhong, B. et al. (2012) Nature immunology 13, 1110-7. PMID: 23042150 8. Wen, J. et al. (2019) Molecular immunology 106, 53-62. PMID: 30579117 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human USP25 Protein, N-His
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
