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| 카탈로그 번호 | HV097012 |
|---|---|
| 설명 |
Recombinant Human USP7 Protein, N-His (HV097012) expressed in E. coli, spanning Gly202-Asn1102. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q93009 |
| 단백질 길이 | Gly202-Asn1102 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 107.31 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Ubiquitin-specific-processing protease 7, Deubiquitinating enzyme 7, Ubiquitin thioesterase 7, USP7, Herpesvirus-associated ubiquitin-specific protease, HAUSP, Ubiquitin carboxyl-terminal hydrolase 7 |
| 배경 | Ubiquitin carboxyl-terminal hydrolase 7 (USP7) is a ~128 kDa protein. Hydrolase that deubiquitinates target proteins such as ARMC5, FOXO4, DEPTOR, KAT5, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5 and DAXX. Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis. Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. 1. Li, M. et al. (2002) Nature 416, 648-53. PMID: 11923872 2. Li, M. et al. (2004) Molecular cell 13, 879-86. PMID: 15053880 3. van der Horst, A. et al. (2006) Nature cell biology 8, 1064-73. PMID: 16964248 4. Song, MS. et al. (2008) Nature 455, 813-7. PMID: 18716620 5. Zhang, J. et al. (2014) Nature communications 5, 5059. PMID: 25283148 6. Cui, H. et al. (2015) Nature communications 6, 6752. PMID: 25865756 7. Ding, X. et al. (2015) PloS one 10, e0145023. PMID: 26678539 8. Jiang, L. et al. (2017) The Journal of biological chemistry 292, 13296-13311. PMID: 28655758 9. Yan, G. et al. (2021) Journal of cellular and molecular medicine 25, 3149-3159. PMID: 33544460 10. Vega, M. et al. (2022) The Journal of biological chemistry 298, 101750. PMID: 35216969 |
| 참고사항 | For research use only. |
SDS-PAGE for Human USP7 Recombinant Protein (N-His)
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