Anti-Human USP7 IP Recombinant Antibody (SAA0238) (HV097013)

Name: Anti-Human USP7 IP Recombinant Antibody (SAA0238)
Brand: abinScience
SKU: HV097013
Price: 191 USD
Availability: InStock

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50ug
100ug
1mg

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HV097013

Species reactivity

Human

Applications

ELISA, IF, IP, IP-MS

Host species

Mouse

Isotype

IgG

Clone ID

SAA0238

Clonality

Monoclonal

Target

Ubiquitin-specific-processing protease 7, Deubiquitinating enzyme 7, Ubiquitin thioesterase 7, USP7, Herpesvirus-associated ubiquitin-specific protease, HAUSP, Ubiquitin carboxyl-terminal hydrolase 7

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q93009

RRID

Anti-Human USP7 IP Antibody (SAA0238) (abinScience Cat# HV097013, RRID:AB_3729772)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Ubiquitin carboxyl-terminal hydrolase 7 (USP7) is a ~128 kDa protein. Hydrolase that deubiquitinates target proteins such as ARMC5, FOXO4, DEPTOR, KAT5, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5 and DAXX. Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis. Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity.

1. Li, M. et al. (2002) Nature 416, 648-53. PMID: 11923872

2. Li, M. et al. (2004) Molecular cell 13, 879-86. PMID: 15053880

3. van der Horst, A. et al. (2006) Nature cell biology 8, 1064-73. PMID: 16964248

4. Song, MS. et al. (2008) Nature 455, 813-7. PMID: 18716620

5. Zhang, J. et al. (2014) Nature communications 5, 5059. PMID: 25283148

6. Cui, H. et al. (2015) Nature communications 6, 6752. PMID: 25865756

7. Ding, X. et al. (2015) PloS one 10, e0145023. PMID: 26678539

8. Jiang, L. et al. (2017) The Journal of biological chemistry 292, 13296-13311. PMID: 28655758

9. Yan, G. et al. (2021) Journal of cellular and molecular medicine 25, 3149-3159. PMID: 33544460

10. Vega, M. et al. (2022) The Journal of biological chemistry 298, 101750. PMID: 35216969

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human USP7 IP Antibody (SAA0238)

References

Datasheet

Anti-Human USP7 IP Recombinant Antibody (SAA0238).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human USP7 IP Recombinant Antibody (SAA0238) (HV097013)

Overview

Images

References

Datasheet

SDS

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