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| 카탈로그 번호 | HD292012 |
|---|---|
| 설명 |
Recombinant Human WIPI1 Protein, N-His (HD292012) expressed in E. coli, spanning Leu17-Leu360. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q5MNZ9 |
| 단백질 길이 | Leu17-Leu360 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 40.44 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Atg18 protein homolog, WD repeat domain phosphoinositide-interacting protein 1, WD40 repeat protein interacting with phosphoinositides of 49 kDa, WIPI 49 kDa, WIPI-1, WIPI1, WIPI49 |
| 배경 | WD repeat domain phosphoinositide-interacting protein 1 is a ~48 kDa protein. Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Plays an important role in starvation- and calcium-mediated autophagy, as well as in mitophagy. Functions downstream of the ULK1 and PI3-kinases that produce phosphatidylinositol 3-phosphate (PtdIns3P) on membranes of the endoplasmic reticulum once activated. Binds phosphatidylinositol 3-phosphate (PtdIns3P), and maybe other phosphoinositides including PtdIns3,5P2 and PtdIns5P, and is recruited to phagophore assembly sites at the endoplasmic reticulum membranes. There, it assists WIPI2 in the recruitment of ATG12-ATG5-ATG16L1, a complex that directly controls the elongation of the nascent autophagosomal membrane. 1. Proikas-Cezanne, T. et al. (2004) Oncogene 23, 9314-25. PMID: 15602573 2. Grotemeier, A. et al. (2010) Cellular signalling 22, 914-25. PMID: 20114074 3. Geeraert, C. et al. (2010) The Journal of biological chemistry 285, 24184-94. PMID: 20484055 4. Itakura, E. et al. (2010) Autophagy 6, 764-76. PMID: 20639694 5. Gaugel, A. et al. (2012) Journal of molecular signaling 7, 16. PMID: 23088497 6. Bakula, D. et al. (2017) Nature communications 8, 15637. PMID: 28561066 7. Maeda, S. et al. (2019) eLife 8. PMID: 31271352 8. Kojima, W. et al. (2021) Autophagy 17, 2011-2036. PMID: 33499712 |
| 참고사항 | For research use only |
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