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| 카탈로그 번호 | HC509012 |
|---|---|
| 설명 |
Recombinant Human XRCC4 Protein, N-His (HC509012) expressed in E. coli, spanning Met1-Lys115. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q13426 |
| 단백질 길이 | Met1-Lys115 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 15.17 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | DNA repair protein XRCC4, X-ray repair cross-complementing protein 4, XRCC4 |
| 배경 | DNA repair protein XRCC4 is a ~38 kDa protein. DNA non-homologous end joining (NHEJ) core factor, required for double-strand break repair and V(D)J recombination. Acts as a scaffold protein that regulates recruitment of other proteins to DNA double-strand breaks (DSBs). Associates with NHEJ1/XLF to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired. The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other. The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors. 1. Nick McElhinny, SA. et al. (2000) Molecular and cellular biology 20, 2996-3003. PMID: 10757784 2. Chen, L. et al. (2000) The Journal of biological chemistry 275, 26196-205. PMID: 10854421 3. Lee, JW. et al. (2003) Cancer research 63, 22-4. PMID: 12517771 4. Foster, RE. et al. (2006) Biochemical and biophysical research communications 341, 175-83. PMID: 16412978 5. Mari, PO. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 18597-602. PMID: 17124166 6. Gu, J. et al. (2007) The EMBO journal 26, 1010-23. PMID: 17290226 7. Roy, S. et al. (2012) Nucleic acids research 40, 1684-94. PMID: 22228831 8. Wanotayan, R. et al. (2015) Biochemical and biophysical research communications 457, 526-31. PMID: 25597996 9. de Bruin, C. et al. (2015) The Journal of clinical endocrinology and metabolism 100, E789-98. PMID: 25742519 10. Fukuchi, M. et al. (2015) Biochemical and biophysical research communications 461, 687-94. PMID: 25934149 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human XRCC4 Protein, N-His
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