Anti-Human XRCC4 Polyclonal Antibody (HC509014)

Name: Anti-Human XRCC4 Polyclonal Antibody
Brand: abinScience
SKU: HC509014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HC509014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human XRCC4 (Met1-Lys115).

Target

DNA repair protein XRCC4, X-ray repair cross-complementing protein 4, XRCC4

Purification

Purified by antigen affinity column.

Accession

Q13426

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA repair protein XRCC4 is a ~38 kDa protein. DNA non-homologous end joining (NHEJ) core factor, required for double-strand break repair and V(D)J recombination. Acts as a scaffold protein that regulates recruitment of other proteins to DNA double-strand breaks (DSBs). Associates with NHEJ1/XLF to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired. The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other. The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors.

1. Nick McElhinny, SA. et al. (2000) Molecular and cellular biology 20, 2996-3003. PMID: 10757784

2. Chen, L. et al. (2000) The Journal of biological chemistry 275, 26196-205. PMID: 10854421

3. Lee, JW. et al. (2003) Cancer research 63, 22-4. PMID: 12517771

4. Foster, RE. et al. (2006) Biochemical and biophysical research communications 341, 175-83. PMID: 16412978

5. Mari, PO. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 18597-602. PMID: 17124166

6. Gu, J. et al. (2007) The EMBO journal 26, 1010-23. PMID: 17290226

7. Roy, S. et al. (2012) Nucleic acids research 40, 1684-94. PMID: 22228831

8. Wanotayan, R. et al. (2015) Biochemical and biophysical research communications 457, 526-31. PMID: 25597996

9. de Bruin, C. et al. (2015) The Journal of clinical endocrinology and metabolism 100, E789-98. PMID: 25742519

10. Fukuchi, M. et al. (2015) Biochemical and biophysical research communications 461, 687-94. PMID: 25934149

Note

For research use only.

Images

References

Datasheet

Anti-Human XRCC4 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human XRCC4 Polyclonal Antibody (HC509014)

Overview

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References

Datasheet

SDS

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