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| 카탈로그 번호 | HV828012 |
|---|---|
| 설명 |
Recombinant Human ZRANB3 Protein, N-His (HV828012) expressed in E. coli, spanning Asp27-Arg256. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q5FWF4 |
| 단백질 길이 | Asp27-Arg256 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 28.98 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Annealing helicase 2, ZRANB3, Zinc finger Ran-binding domain-containing protein 3, AH2, DNA annealing helicase and endonuclease ZRANB3 |
| 배경 | DNA annealing helicase and endonuclease ZRANB3 is a ~123 kDa protein. DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks. 1. Yusufzai, T. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 20970-3. PMID: 21078962 2. Ciccia, A. et al. (2012) Molecular cell 47, 396-409. PMID: 22704558 3. Yuan, J. et al. (2012) Molecular cell 47, 410-21. PMID: 22705370 4. Weston, R. et al. (2012) Genes & development 26, 1558-72. PMID: 22759634 5. Badu-Nkansah, A. et al. (2016) The Journal of biological chemistry 291, 8251-7. PMID: 26884333 6. Sebesta, M. et al. (2017) Nature communications 8, 15847. PMID: 28621305 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human ZRANB3 Protein, N-His
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