Anti-Human ZRANB3 Polyclonal Antibody (HV828014)

Name: Anti-Human ZRANB3 Polyclonal Antibody
Brand: abinScience
SKU: HV828014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HV828014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ZRANB3 (Asp27-Arg256).

Target

Annealing helicase 2, ZRANB3, Zinc finger Ran-binding domain-containing protein 3, AH2, DNA annealing helicase and endonuclease ZRANB3

Purification

Purified by antigen affinity column.

Accession

Q5FWF4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA annealing helicase and endonuclease ZRANB3 is a ~123 kDa protein. DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks.

1. Yusufzai, T. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 20970-3. PMID: 21078962

2. Ciccia, A. et al. (2012) Molecular cell 47, 396-409. PMID: 22704558

3. Yuan, J. et al. (2012) Molecular cell 47, 410-21. PMID: 22705370

4. Weston, R. et al. (2012) Genes & development 26, 1558-72. PMID: 22759634

5. Badu-Nkansah, A. et al. (2016) The Journal of biological chemistry 291, 8251-7. PMID: 26884333

6. Sebesta, M. et al. (2017) Nature communications 8, 15847. PMID: 28621305

Note

For research use only.

Images

References

Datasheet

Anti-Human ZRANB3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Anti-Human ZRANB3 Polyclonal Antibody (HV828014)

Overview

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References

Datasheet

SDS

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