Mouse LAG-3 Recombinant Protein (C-Fc) (MB613021)

Name: Mouse LAG-3 Recombinant Protein (C-Fc)
Brand: abinScience
SKU: MB613021
Price: 298 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

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50ug
100ug
1mg

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개요
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참고 문헌
데이터시트
SDS

개요

카탈로그 번호	MB613021
설명	Recombinant Mouse CD223/LAG3 Protein, C-Fc (MB613021) expressed in Mammalian Cells, spanning Gly24-Leu442. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	Mammalian cells
등록번호	Q61790
단백질 길이	Gly24-Leu442
응용 분야	SDS-PAGE, WB, ELISA, Immunogen, Bioactivity testing in progress
종	Mus musculus (Mouse)
성질	Recombinant
타겟	CD223, FDC, LAG-3, LAG3, Lymphocyte activation gene 3 protein, Secreted lymphocyte activation gene 3 protein, sLAG-3
내독소 수준	Please contact with the lab for this information.
순도	>95% as determined by SDS-PAGE.
예측 분자량	73.58 kDa
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	CD223, FDC, LAG-3, LAG3, Lymphocyte activation gene 3 protein, Secreted lymphocyte activation gene 3 protein, sLAG-3
배경	Lymphocyte activation gene 3 protein (CD223) is a ~56 kDa protein. Inhibitory receptor on antigen activated T-cells. Delivers inhibitory signals upon binding to ligands, such as MHC class II, its main ligand present at the surface of antigen-presenting cells (APCs), and FGL1, which is secreted by hepatocytes and certain types of tumor cells. Ligand-binding initiates a signaling that inhibits the T-cell receptor (TCR) in the immunological synapse, preventing T-cell activation. Mechanistically, ligand-binding promotes (1) ubiquitination of the KIEELE motif, unleashing the RRFSALE motif from the membrane and (2) leading to the formation of condensates with the TCR component CD3E, thereby disrupting the association between CD3E and LCK and preventing TCR activation. May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1. 1. Workman, CJ. et al. (2002) European journal of immunology 32, 2255-63. PMID: 12209638 2. Workman, CJ. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 169, 5392-5. PMID: 12421911 3. Workman, CJ. et al. (2003) European journal of immunology 33, 970-9. PMID: 12672063 4. Workman, CJ. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 172, 5450-5. PMID: 15100286 5. Workman, CJ. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 174, 688-95. PMID: 15634887 6. Wang, J. et al. (2019) Cell 176, 334-347.e12. PMID: 30580966 7. Maruhashi, T. et al. (2022) Immunity 55, 912-924.e8. PMID: 35413245 8. Ming, Q. et al. (2022) Nature immunology 23, 1031-1041. PMID: 35761082 9. Silberstein, JL. et al. (2024) Proceedings of the National Academy of Sciences of the United States of America 121, e2310866121. PMID: 38483996 10. Ming, Q. et al. (2024) Nature communications 15, 7513. PMID: 39209860
참고사항	For research use only.