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Mouse CD8 alpha Recombinant Protein (C-His) (MF815011)

Mouse CD8 alpha Recombinant Protein (C-His)
Mouse CD8 alpha Recombinant Protein (C-His)
Mouse CD8 alpha Recombinant Protein (C-His)
Mouse CD8 alpha Recombinant Protein (C-His)
Mouse CD8 alpha Recombinant Protein (C-His)
Mouse CD8 alpha Recombinant Protein (C-His)
가격(USD):
사양:
  • 50ug
  • 100ug
  • 1mg
수량:
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  • 개요

  • 이미지

  • 참고 문헌

  • 데이터시트

  • SDS

개요
카탈로그 번호MF815011
설명
Recombinant Mouse CD8A Protein, C-His (MF815011) expressed in Mammalian Cells, spanning Lys28-Tyr196. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템Mammalian cells
등록번호P01731
단백질 길이Lys28-Tyr196
응용 분야ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종Mus musculus (Mouse)
성질 Recombinant
타겟 CD8a, Cd8a, Lyt-2, Lyt2, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2
내독소 수준 Please contact with the lab for this information.
순도 >90% as determined by SDS-PAGE.
예측 분자량 21.78 kDa
형태 Lyophilized
저장 완충액 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

재구성 Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름CD8a, Cd8a, Lyt-2, Lyt2, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2
배경

T-cell surface glycoprotein CD8 alpha chain (CD8a/Lyt2) is a ~27 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.

참고사항 For research use only.
이미지
  • Mouse CD8 alpha Recombinant Protein (C-His)

    Bioactivity

    Detects Mouse CD8a/Lyt2 Antibody (YTS105.18) in indirect ELISAs.

  • Mouse CD8 alpha Recombinant Protein (C-His)

    Bioactivity

    Detects Mouse CD8a/Lyt2 Antibody (2.43) in indirect ELISAs.

  • Mouse CD8 alpha Recombinant Protein (C-His)

    Bioactivity

    Detected by Anti-Mouse CD8a/Lyt2 Antibody (53-6.72) (Catalog No: MF815607) in indirect ELISA

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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