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Mouse DGAT1 Recombinant Protein (N-GST & C-His) (MF844012)

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사양:
  • 50ug
  • 100ug
  • 1mg
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  • 개요

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  • 데이터시트

  • SDS

개요
카탈로그 번호MF844012
설명
Recombinant Mouse DGAT1 Protein, N-GST & C-His (MF844012) expressed in E. coli, spanning Gly2-Ser92. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템E. coli
등록번호Q9Z2A7
단백질 길이Gly2-Ser92
응용 분야ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종Mus musculus (Mouse)
성질 Recombinant
내독소 수준 Please contact with the lab for this information.
순도 >90% as determined by SDS-PAGE.
예측 분자량 37.58 kDa
형태 Lyophilized
저장 완충액 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

재구성 Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
배송 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름ACAT-related gene product 1, AGRP1, ARAT, Acyl-CoA retinol O-fatty-acyltransferase, DGAT, DGAT1, Diacylglycerol O-acyltransferase 1, Diglyceride acyltransferase, EC:2.3.1.20, EC:2.3.1.76, Retinol O-fatty-acyltransferase
배경

Diacylglycerol O-acyltransferase 1 is a ~56 kDa protein. Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats. In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage. Also present in female mammary glands, where it produces fat in the milk. May be involved in VLDL (very low density lipoprotein) assembly.

1. Yen, CL. et al. (2005) Journal of lipid research 46, 1502-11. PMID: 15834126
2. Shih, MY. et al. (2009) The Journal of biological chemistry 284, 4292-9. PMID: 19028692
3. McFie, PJ. et al. (2010) The Journal of biological chemistry 285, 37377-87. PMID: 20876538
4. Qi, J. et al. (2012) Journal of lipid research 53, 1106-16. PMID: 22493088
5. Ma, Z. et al. (2017) Journal of lipid research 58, 1091-1099. PMID: 28420705
6. Buhman, KK. et al. (2002) The Journal of biological chemistry 277, 25474-9. PMID: 11959864
참고사항 For research use only
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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