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Anti-Mouse DGAT1 Polyclonal Antibody (MF844014)

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Overview
Catalog No.MF844014
Description
Anti-Mouse DGAT1 Polyclonal Antibody (MF844014) is a rabbit polyclonal antibody detecting DGAT1. Suitable for Mouse and Rat.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse DGAT1 (Gly2-Ser92).
Target ACAT-related gene product 1, AGRP1, ARAT, Acyl-CoA retinol O-fatty-acyltransferase, DGAT, DGAT1, Diacylglycerol O-acyltransferase 1, Diglyceride acyltransferase, EC:2.3.1.20, EC:2.3.1.76, Retinol O-fatty-acyltransferase
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q9Z2A7
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Diacylglycerol O-acyltransferase 1 (DGAT1) is a ~56 kDa protein. Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats. In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage. Also present in female mammary glands, where it produces fat in the milk. May be involved in VLDL (very low density lipoprotein) assembly.

1. Yen, CL. et al. (2005) Journal of lipid research 46, 1502-11. PMID: 15834126
2. Shih, MY. et al. (2009) The Journal of biological chemistry 284, 4292-9. PMID: 19028692
3. McFie, PJ. et al. (2010) The Journal of biological chemistry 285, 37377-87. PMID: 20876538
4. Qi, J. et al. (2012) Journal of lipid research 53, 1106-16. PMID: 22493088
5. Ma, Z. et al. (2017) Journal of lipid research 58, 1091-1099. PMID: 28420705
6. Buhman, KK. et al. (2002) The Journal of biological chemistry 277, 25474-9. PMID: 11959864
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse DGAT1 Polyclonal Antibody

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