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Mouse DGAT1 Recombinant Protein (N-GST & C-His) (MF844012)

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Overview
Catalog No.MF844012
Description
Recombinant Mouse DGAT1 Protein, N-GST & C-His (MF844012) expressed in E. coli, spanning Gly2-Ser92. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionQ9Z2A7
Protein lengthGly2-Ser92
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesMus musculus (Mouse)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 37.58 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesACAT-related gene product 1, AGRP1, ARAT, Acyl-CoA retinol O-fatty-acyltransferase, DGAT, DGAT1, Diacylglycerol O-acyltransferase 1, Diglyceride acyltransferase, EC:2.3.1.20, EC:2.3.1.76, Retinol O-fatty-acyltransferase
Background

Diacylglycerol O-acyltransferase 1 is a ~56 kDa protein. Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats. In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage. Also present in female mammary glands, where it produces fat in the milk. May be involved in VLDL (very low density lipoprotein) assembly.

1. Yen, CL. et al. (2005) Journal of lipid research 46, 1502-11. PMID: 15834126
2. Shih, MY. et al. (2009) The Journal of biological chemistry 284, 4292-9. PMID: 19028692
3. McFie, PJ. et al. (2010) The Journal of biological chemistry 285, 37377-87. PMID: 20876538
4. Qi, J. et al. (2012) Journal of lipid research 53, 1106-16. PMID: 22493088
5. Ma, Z. et al. (2017) Journal of lipid research 58, 1091-1099. PMID: 28420705
6. Buhman, KK. et al. (2002) The Journal of biological chemistry 277, 25474-9. PMID: 11959864
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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