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| 카탈로그 번호 | JN038012 |
|---|---|
| 설명 |
Recombinant Treponema pallidum/TP TPP47 Protein, C-His (JN038012) expressed in E. coli, spanning Cys20-Gln434. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P29723 |
| 단백질 길이 | Cys20-Gln434 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Treponema pallidum |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 47.48 kDa |
| 형태 | Liquid |
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Putative DD-carboxypeptidase TP_0574, 3.4.-.-, 47 kDa lipoprotein, Tp47, Tpp47, 47 kDa membrane antigen, 47-kilodalton major integral membrane immunogen, TP_0574 |
| 배경 | Putative DD-carboxypeptidase TP_0574 (TPP47) is a ~47 kDa protein. A possible D,D-carboxypeptidase, that releases amino acids sequentially from a proteins C-terminus. Has zinc-dependent carboxypeptidase activity on synthetic depsipeptide substrates. May serve to decrease cross-linking of peptidoglycan, promoting the highly sinusous motility of this spirochaete (Probable). Overexpression of the whole protein in E.coli leads to aberrant cell morphology and extrusion of the cytoplasm, while overexpression of a construct with the first 62 resides of the protein fused to PhoA does have this effect, suggesting the whole protein, not the lipoprotein moiety, is toxic. Binds penicillin. 1. Deka, RK. et al. (2002) The Journal of biological chemistry 277, 41857-64. PMID: 12196546 2. Weigel, LM. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 11611-5. PMID: 7972112 3. Radolf, JD. et al. (1989) Infection and immunity 57, 1248-54. PMID: 2647634 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Treponema pallidum/TP TPP47 Protein, C-His
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중국 우한시 심둔사로 666번지 C동, 우한, 430206
