Anti-Treponema pallidum TPP47 Polyclonal Antibody (JN038014)

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Overview

Catalog No.

JN038014

Description

Anti-Treponema pallidum TPP47 Polyclonal Antibody (JN038014) is a rabbit polyclonal antibody detecting Treponema Pallidum (TP in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Treponema pallidum (strain Nichols)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Treponema pallidum TPP47 (Cys20-Gln434).

Target

Putative DD-carboxypeptidase TP_0574, 3.4.-.-, 47 kDa lipoprotein, Tp47, Tpp47, 47 kDa membrane antigen, 47-kilodalton major integral membrane immunogen, TP_0574, Treponema Pallidum (TP, Syphilis)

Purification

Purified by antigen affinity column.

Accession

P29723

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Putative DD-carboxypeptidase TP_0574 (TPP47) is a ~47 kDa protein. A possible D,D-carboxypeptidase, that releases amino acids sequentially from a proteins C-terminus. Has zinc-dependent carboxypeptidase activity on synthetic depsipeptide substrates. May serve to decrease cross-linking of peptidoglycan, promoting the highly sinusous motility of this spirochaete (Probable). Overexpression of the whole protein in E.coli leads to aberrant cell morphology and extrusion of the cytoplasm, while overexpression of a construct with the first 62 resides of the protein fused to PhoA does have this effect, suggesting the whole protein, not the lipoprotein moiety, is toxic. Binds penicillin.

1. Deka, RK. et al. (2002) The Journal of biological chemistry 277, 41857-64. PMID: 12196546

2. Weigel, LM. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 11611-5. PMID: 7972112

3. Radolf, JD. et al. (1989) Infection and immunity 57, 1248-54. PMID: 2647634

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Treponema pallidum TPP47 polyclonal antibody at 1ug/mL on recombinant Treponema pallidum TPP47 (Catalog No. JN038012).

References

Datasheet

Anti-Treponema pallidum TPP47 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Treponema pallidum TPP47 Polyclonal Antibody (JN038014)

Overview

Images

References

Datasheet

SDS

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