Anti-Human TNF RI/TNFRSF1A Reference Antibody (GSK1995057, RUO) (HB895036)

Name: Anti-Human TNF RI/TNFRSF1A Reference Antibody (GSK1995057, RUO)
Brand: abinScience
SKU: HB895036
Price: 176 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

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100ug
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5mg
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개요
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참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HB895036

종 반응성

Human

응용 분야

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

숙주 종

Human

클론성

Monoclonal

이소타입

VHH-His

클론 ID

GSK1995057

생물학적 활성

GSK1995057 (10 nM; 12 h) significantly inhibits LPS ()-induced loss of cell viability in human nucleus pulposus cells, and attenuates cell apoptosis by regulating proteins of the extrinsic apoptotic pathway [2]. GSK1995057 (10 nM; 12 h) significantly inhibits LPS-induced secretion of pro-inflammatory and regulatory cytokines (IL-1β, IL-6, IL-10, TNF-α), suppresses the upregulated expression of extracellular matrix degrading enzyme genes (MMP-1, MMP-3, MMP-13, ADAMTS-4, ADAMTS-5), and blocks the activation of NF-κB, JNK and P38 MAPK signaling pathways in human nucleus pulposus cells [2]. GSK1995057 (10 nM; 1-4 h) potently inhibits TNF-α-stimulated neutrophil-endothelial cell interactions, endothelial permeability, adhesion molecule expression, and proinflammatory cytokine release in human lung microvascular endothelial cells (HMVEC-L) and human neutrophils [3]. GSK1995057 (0.00001-1 uM; 1 h serum incubation, 24 h cell incubation), upon binding to serum from anti-HAVH antibody-positive donors, activates TNFR1 and induces IL-8 release from MRC-5 human lung fibroblasts, human alveolar epithelial cells, and human primary lung fibroblasts in a concentration-dependent bell-shaped manner [4]. GSK1995057 binds with high affinity (1.65×10 -10 M) to purified polyclonal HAVH autoantibodies [4]. GSK1995057 (0.00001-1 uM) induces TNF-R1 activation and IL-8 release in the human lung fibroblast cell line MRC-5 when mixed with human serum samples containing the co-existing specific autoantibodies against GSK2862277, with the activation effect peaking at 0.001 uM [5].

발현 시스템

Mammalian cells

타겟

TNFR-I, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, TBPI, p60, CD120a, TNFRSF1A, Tumor necrosis factor receptor type I, p55, TNF-RI, TNFAR, TNFR1, TNF-R1

내독소 수준

< 10 EU/mg

순도

>95% purity as determined by SDS-PAGE.

정제

Purified by Nickel column

등록번호

P19438

형태

Liquid

저장 완충액

0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:2000-1:20000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

대체 이름

GSK1995057

배경

Tumor necrosis factor receptor superfamily member 1A (CD120a/TNFR1) is a ~50 kDa protein. Receptor for TNFSF2/TNF and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.

참고 문헌

1. Liao K, et al. Detection of Memory B Activity Against a Therapeutic Protein in Treatment-Naïve Subjects. AAPS J. 2018;20(3):51. Published 2018 Mar 16. [HB895036]
2. Lv F, et al. Inhibition of TNFR1 Attenuates LPS Induced Apoptosis and Inflammation in Human Nucleus Pulposus Cells by Regulating the NF-KB and MAPK Signalling Pathway. Neurochem Res. 2021;46(6):1390-1399. [HB895036]
3. Proudfoot A, et al. Novel anti-tumour necrosis factor receptor-1 (TNFR1) domain antibody prevents pulmonary inflammation in experimental acute lung injury. Thorax. 2018;73(8):723-730. [HB895036]
4. Holland MC, et al. Autoantibodies to variable heavy (VH) chain Ig sequences in humans impact the safety and clinical pharmacology of a VH domain antibody antagonist of TNF-α receptor 1. J Clin Immunol. 2013;33(7):1192-1203. [HB895036]
5. Cordy JC, et al. Specificity of human anti-variable heavy (VH) chain autoantibodies and impact on the design and clinical testing of a VH domain antibody antagonist of tumour necrosis factor-α receptor 1. Clin Exp Immunol. 2015;182(2):139-148. View More [HB895036]

참고사항

For research use only. Not suitable for clinical or therapeutic use.

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