Anti-Human TNF RI/TNFRSF1A Reference Antibody (ATROSAB, RUO) (HB895026)

Name: Anti-Human TNF RI/TNFRSF1A Reference Antibody (ATROSAB, RUO)
Brand: abinScience
SKU: HB895026
Price: 176 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

100ug
1mg
5mg
10mg
25mg
50mg
100mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HB895026
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-kappa
Clone ID	ATROSAB
Biological activity	Atrosab (0.1-1000 nM, preincubate with antibody for 1 h, then add the stimulant TNF and co-incubate for 7 hours) inhibits in a dose-dependent manner the TNF-induced apoptosis of Kym-1 cells [1]. Atrosab (0.1-1000 nM, preincubate with antibody for 1 h, then add the stimulant TNF and co-incubate for 7 hours) inhibits release of IL-6 from HeLa cells and IL-8 from HT1080 cells induced by TNF dose-dependently [1]. Atrosab (100 ug/mL,24 h) reduces endothelial cell expression of adhesion molecules and T cell adhesion [3]. . Inhibited IL-6 release from HeLa cells with an IC 50 of 60 nM. Inhibited IL-8 release from HT1080 cells with an IC 50 of 60 nM. Atrosab (i.v., twice weekly, 28 days) has been shown to be effective for inflammatory diseases such as rheumatoid arthritis (RA) and multiple sclerosis (MS) in both monkeys and human TNF transgenic/human TNFR1 knock-in mice model and in human TNFR1 knock-in mice (hp55K/i) EAE model [2]. Atrosab (20 mg/kg, i.p.) reduces immune cell infiltrates in humanized TNFR1 knock-in mice [3].
Expression system	Mammalian cells
Clonality	Monoclonal
Target	TNFR-I, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, TBPI, p60, CD120a, TNFRSF1A, Tumor necrosis factor receptor type I, p55, TNF-RI, TNFAR, TNFR1, TNF-R1
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P19438
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ATROSAB
Background	Tumor necrosis factor receptor superfamily member 1A (CD120a/TNFR1) is a ~50 kDa protein. Receptor for TNFSF2/TNF and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
References	1. Zettlitz KA, et al. ATROSAB, a humanized antagonistic anti-tumor necrosis factor receptor one-specific antibody. MAbs. 2010 Nov-Dec;2(6):639-47. [HB895026] 2. Zettlitz KA, et al. ATROSAB, a humanized antagonistic anti-tumor necrosis factor receptor one-specific antibody. [HB895026] 3. E. Guenzi, et al. FRI0231 Atrosab, a humanized antibody directed against tnf-receptor 1, hold great promises for the treatment of rheumatoid arthritis. Ann Rheum Dis. 2013 Jun 14; 72(3):A451. [HB895026] 4. Williams SK, et al. Anti-TNFR1 targeting in humanized mice ameliorates disease in a model of multiple sclerosis. Sci Rep. 2018 Sep 11;8(1):13628. View More [HB895026]
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

Bioactivity

Detects Recombinant Human CD120a/TNFRSF1A Protein, C-His (Cat No.: HB895011) in indirect ELISA.
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD120a/TNFRSF1A/TNFR1 (ATROSAB).