Anti-Human HGFR/c-MET Reference Antibody (CE-355621, RUO) (HY196056)

Name: Anti-Human HGFR/c-MET Reference Antibody (CE-355621, RUO)
Brand: abinScience
SKU: HY196056
Price: 370 USD
Availability: InStock

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개요

카탈로그 번호	HY196056
종 반응성	Human
응용 분야	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
숙주 종	Human
클론성	Monoclonal
이소타입	IgG
클론 ID	CE-355621
생물학적 활성	CE-355621 (0.01-100 nM, 4 h) can efficiently block the binding of c-Met to HGF and induce c-Met degradation in A549 cells [1]. CE-355621 (0.01 nM-10 nM, 24 h) significantly inhibits c-Met activation in U87MG cells [1]. CE-355621 (0.01-10 ug/mL, 48 h) can induce c-Met degradation in U87MG cells [1]. Western Blot Analysis [1] Cell Line: U87MG cells Concentration: 0.01 nM-10 nM Incubation Time: 24 h Result: Significantly inhibited activation of c-Met. Western Blot Analysis [1] Cell Line: U87MG cells Concentration: 0.01-10 ug/mL Incubation Time: 48 h Result: Reduced the level of c-Met. CE-355621 (25-200 ug, i.p., on day 0, 2, 3, 6 and 8) significantly delays tumor growth in female CD-1 nu/nu mice bearing U87MG xenografts [1]. CE-355621 (400 ug, i.p., on day 7 and 14) significantly inhibits tumor growth in female CD-1 nu/nu mice bearing GTL-16 xenografts [1]. CE-355621 (200 ug, i.p., single dose, on day 7) can effectively inhibit tumor volume growth and uptake of 18 F-FDG in female nude (nu/nu) mice bearing U87MG xenografts [2].
발현 시스템	Mammalian cells
타겟	Tyrosine-protein kinase Met, Hepatocyte growth factor receptor, Proto-oncogene c-Met, Scatter factor receptor, HGF receptor, SF receptor, HGF/SF receptor, MET
내독소 수준	< 10 EU/mg
순도	>95% purity as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	P08581
형태	Liquid
저장 완충액	0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	CE-355621, CE355621, CE355621
배경	Hepatocyte growth factor receptor (MET/HGFR) is a ~155 kDa protein. Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. MET is the therapeutic target of amivantamab (Rybrevant).
참고 문헌	1. Michaud NR, et al. Biochemical and pharmacological characterization of human c-Met neutralizing monoclonal antibody CE-355621. MAbs. 2012 Nov-Dec;4(6):710-23. [HY196056] 2. Tseng JR, et al. Preclinical efficacy of the c-Met inhibitor CE-355621 in a U87 MG mouse xenograft model evaluated by 18F-FDG small-animal PET. J Nucl Med. 2008 Jan;49(1):129-134. [HY196056]
참고사항	For research use only. Not suitable for clinical or therapeutic use.