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Anti-Human TNF-alpha Reference Antibody (Golimumab, RUO) (HF879046)

Anti-Human TNF-alpha Reference Antibody (Golimumab, RUO)
Anti-Human TNF-alpha Reference Antibody (Golimumab, RUO)
Anti-Human TNF-alpha Reference Antibody (Golimumab, RUO)
Anti-Human TNF-alpha Reference Antibody (Golimumab, RUO)
Anti-Human TNF-alpha Reference Antibody (Golimumab, RUO)
Anti-Human TNF-alpha Reference Antibody (Golimumab, RUO)
Anti-Human TNF-alpha Reference Antibody (Golimumab, RUO)
Anti-Human TNF-alpha Reference Antibody (Golimumab, RUO)
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사양:
  • 100ug
  • 1mg
  • 5mg
  • 10mg
  • 25mg
  • 50mg
  • 100mg
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  • 개요

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  • 참고 문헌

  • 데이터시트

  • SDS

개요
카탈로그 번호HF879046
설명
Golimumab (HF879046) is a research-grade recombinant antibody targeting CD2. Produced in mammalian cells with native-like glycosylation.
Highlights
  • ●Research Grade — For PK/PD studies, assay development, and ADA research.
  • ●Native Glycosylation — Mammalian expression ensures native-like patterns.
종 반응성Human
응용 분야ELISA, Bioactivity: FCM, Functional assay, Research in vivo
숙주 종Human
클론성Monoclonal
이소타입IgG1-kappa
테스트된 응용 분야 FCM
타겟 C-domain 1, C-domain 2, Cachectin, ICD1, ICD2, Intracellular domain 1, Intracellular domain 2, N-terminal fragment, NTF, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form
내독소 수준 < 10 EU/mg
순도 >95% as determined by SDS-PAGE.
정제 Protein A/G purified from cell culture supernatant.
등록번호 P01375
형태 Liquid
저장 완충액 0.01M PBS pH 7.4.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름CNTO148, 476181-74-5
배경Onercept is a recombinant soluble human tumor necrosis factor-alpha p55 receptor for use in Crohn's disease research.
참고사항 For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
이미지
  • Anti-Human TNF-alpha Reference Antibody (Golimumab, RUO)

    SEC-HPLC

    SEC-HPLC detection for Research Grade Golimumab.

  • Anti-Human TNF-alpha Reference Antibody (Golimumab, RUO)

    Bioactivity

    Detects Human TNFa/TNF-alpha in indirect ELISAs.

  • Anti-Human TNF-alpha Reference Antibody (Golimumab, RUO)

    SDS-PAGE

    SDS-PAGE for Research Grade Golimumab

  • Anti-Human TNF-alpha Reference Antibody (Golimumab, RUO)

    Flow CytoMetry

    Flow-cytometry using anti-human TNFa antibody. 293T Transtected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa monoclonal antibody (Catalog: HF879046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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