Anti-Human IL-5 Reference Antibody (Mepolizumab, RUO) (HY218026)

Name: Anti-Human IL-5 Reference Antibody (Mepolizumab, RUO)
Brand: abinScience
SKU: HY218026
Price: 176 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

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100ug
1mg
5mg
10mg
25mg
50mg
100mg

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HY218026
설명	Mepolizumab (HY218026) is a research-grade recombinant antibody targeting IL5. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
종 반응성	Human
응용 분야	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
숙주 종	Human
클론성	Monoclonal
이소타입	IgG1-kappa
발현 시스템	Mammalian cells
테스트된 응용 분야	WB
타겟	IL5, B-cell differentiation factor I, IL-5, T-cell replacing factor, TRF, Eosinophil differentiation factor, Interleukin-5
내독소 수준	< 10 EU/mg
순도	>95% purity as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	P05113
형태	Liquid
저장 완충액	0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	SB-240563, 196078-29-2
배경	Interleukin-5 (IL5) is a ~15 kDa protein. Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively. IL5 is the therapeutic target of mepolizumab (Nucala). 1. Clutterbuck, EJ. et al. (1989) Blood 73, 1504-12. PMID: 2653458 2. Ochiai, K. et al. (1997) Clinical and experimental immunology 107, 198-204. PMID: 9010276 3. Tavernier, J. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 7041-5. PMID: 1495999 4. Kusano, S. et al. (2012) Protein science : a publication of the Protein Society 21, 850-64. PMID: 22528658 5. Alam, R. et al. (1995) International archives of allergy and immunology 107, 226-7. PMID: 7613138
참고사항	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

이미지

SDS-PAGE

SDS-PAGE for Research Grade Mepolizumab.
Bioactivity

SEC-HPLC detection for Research Grade Mepolizumab.
Western blot

Western blot analysis was performed using anti-IL5 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IL5 (Catalog No: HY218012), 50ng
Lane 2: recombinant human IL5 (Catalog No: HY218012), 30ng
Lane 3: recombinant human IL5 (Catalog No: HY218012), 20ng