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Recombinant Human IL5 Protein, N-His (HY218012)

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Recombinant Human IL5 Protein, N-His

Recombinant Human IL5 Protein, N-His

Recombinant Human IL5 Protein, N-His

Recombinant Human IL5 Protein, N-His

Recombinant Human IL5 Protein, N-His

Recombinant Human IL5 Protein, N-His

Recombinant Human IL5 Protein, N-His

Recombinant Human IL5 Protein, N-His

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Overview
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References
Datasheet

Overview

Catalog No.	HY218012
Description	Recombinant Human IL5 Protein, N-His (HY218012) expressed in E. coli, spanning Ile20-Ser134. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P05113
Protein length	Ile20-Ser134
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	16.31 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	IL5, B-cell differentiation factor I, IL-5, T-cell replacing factor, TRF, Eosinophil differentiation factor, Interleukin-5
Background	Interleukin-5 (IL5) is a ~15 kDa protein. Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively. IL5 is the therapeutic target of mepolizumab (Nucala). 1. Clutterbuck, EJ. et al. (1989) Blood 73, 1504-12. PMID: 2653458 2. Ochiai, K. et al. (1997) Clinical and experimental immunology 107, 198-204. PMID: 9010276 3. Tavernier, J. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 7041-5. PMID: 1495999 4. Kusano, S. et al. (2012) Protein science : a publication of the Protein Society 21, 850-64. PMID: 22528658 5. Alam, R. et al. (1995) International archives of allergy and immunology 107, 226-7. PMID: 7613138
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human IL5 Protein, N-His
Bioactivity

Detected by Research Grade Depemokimab (Cat No.: HY218036) in indirect ELISA.
Western blot

Detected by Anti-IL5 monoclonal antibody (Catalog No: HF572010)
Bioactivity

Detected by Varokibart (Catalog No: HY218046) in indirect ELISA.

References

HY218012-SDS-PAGE-1.jpg

Datasheet

Recombinant Human IL5 Protein, N-His.pdf

Formula

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Recombinant Human IL5 Protein, N-His