3C Protease (VK794012)

Name: 3C Protease
Brand: abinScience
SKU: VK794012
Price: 298 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

1000U
5000U

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	VK794012
설명	3C Protease (VK794012) expressed in E. coli. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	E. coli
단백질 길이	3C Protease is cloned from Human rhinovirus, fused with GST tag and expressed in E.coli.
응용 분야	Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP).
성질	Recombinant
내독소 수준	Please contact with the lab for this information.
순도	>95% as determined by SDS-PAGE.
형태	Liquid
저장 완충액	50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
참고사항	For research use only.

이미지

SDS-PAGE

SDS-PAGE for 3C Protease

Bioactivity

M: Marker
1: Control
2: Fusion protein digest with 3C protease

Case study		Formula
concentration	1 mg/ml	c mg/ml
volume	100 µl	V ml
substrate	100 µg	cV mg
10X Buffer	11 µl	V/9 ml
3C Protease	1 µl	cV/100 ml

Mix the components thoroughly and incubate the reaction at 4°C for 16 hours. After incubation, take 2–10 µg of the control and digested sample for analysis by SDS-PAGE.

참고 문헌

데이터시트