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| 카탈로그 번호 | HV826013 | ||||
|---|---|---|---|---|---|
| 설명 |
Anti-Human ASC/TMS1/PYCARD Nanobody (SAA1187) [SAA1187] (HV826013) is a alpaca monoclonal antibody detecting PYCARD in ELISA, IF, IP. Suitable for Human.
Highlights
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| 종 반응성 | Human | ||||
| 응용 분야 | ELISA, IF, IP | ||||
| 숙주 종 | Alpaca | ||||
| 클론성 | Monoclonal | ||||
| 이소타입 | VHH-8His-Cys-tag | ||||
| 클론 ID | SAA1187 | ||||
| 타겟 | TMS1, CARD5, Caspase recruitment domain-containing protein 5, Apoptosis-associated speck-like protein containing a CARD, PYD and CARD domain-containing protein, ASC, PYCARD, hASC, Target of methylation-induced silencing 1 | ||||
| 내독소 수준 | Please contact with the lab for this information. | ||||
| 순도 | >95% as determined by SDS-PAGE. | ||||
| 정제 | Purified by Nickel column | ||||
| 등록번호 | Q9ULZ3 | ||||
| 형태 | Liquid | ||||
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. | ||||
| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| 참고사항 | For research use only. |
SDS-PAGE for Anti-Human ASC/TMS1/PYCARD Nanobody (SAA1187)
MCF7 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with His-tagged ASC protein (HV826013) at a concentration of 20 µg/mL overnight at 4°C. After washing, the cells were incubated with an anti-His primary antibody for 1 hour at room temperature, followed by incubation with Goat Anti-Mouse IgG (Alexa Fluor 488) for 50 minutes at room temperature. The localization of IDE was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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