Anti-ASC/TMS1/PYCARD Polyclonal Antibody (HV826014)

Name: Anti-ASC/TMS1/PYCARD Polyclonal Antibody
Brand: abinScience
SKU: HV826014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HV826014

Description

Anti-ASC/TMS1/PYCARD Polyclonal Antibody (HV826014) is a rabbit polyclonal antibody detecting PYCARD in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ASC/TMS1/PYCARD (Leu112-Ser195).

Target

TMS1, CARD5, Caspase recruitment domain-containing protein 5, Apoptosis-associated speck-like protein containing a CARD, PYD and CARD domain-containing protein, ASC, PYCARD, hASC, Target of methylation-induced silencing 1

Purification

Purified by antigen affinity column.

Accession

Q9ULZ3

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Apoptosis-associated speck-like protein containing a CARD (ASC/TMS1) is a ~21 kDa protein. Functions as a key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in innate immune response by acting as an integral adapter in the assembly of various inflammasomes (NLRP1, NLRP2, NLRP3, NLRP6, AIM2 and probably IFI16) which recruit and activate caspase-1 leading to processing and secretion of pro-inflammatory cytokines. Caspase-1-dependent inflammation leads to macrophage pyroptosis, a form of cell death.

1. McConnell, BB. et al. (2000) Cancer research 60, 6243-7. PMID: 11103777

2. Masumoto, J. et al. (2003) Biochemical and biophysical research communications 303, 69-73. PMID: 12646168

3. Agostini, L. et al. (2004) Immunity 20, 319-25. PMID: 15030775

4. Faustin, B. et al. (2007) Molecular cell 25, 713-24. PMID: 17349957

5. Fernandes-Alnemri, T. et al. (2007) Cell death and differentiation 14, 1590-604. PMID: 17599095

6. Hornung, V. et al. (2009) Nature 458, 514-8. PMID: 19158675

7. Fernandes-Alnemri, T. et al. (2009) Nature 458, 509-13. PMID: 19158676

8. Bryan, NB. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 3173-82. PMID: 19234215

9. Hasegawa, M. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 7655-62. PMID: 19494289

10. Taxman, DJ. et al. (2011) The Journal of biological chemistry 286, 19605-16. PMID: 21487011

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ASC/TMS1/PYCARD polyclonal antibody at 1ug/mL on recombinant ASC/TMS1/PYCARD (Catalog No. HV826012).

References

Datasheet