Anti-Human ASC/TMS1/PYCARD Recombinant Nanobody (SAA1187) (HV826013)

Name: Anti-Human ASC/TMS1/PYCARD Recombinant Nanobody (SAA1187)
Brand: abinScience
SKU: HV826013
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV826013

Description

Anti-Human ASC/TMS1/PYCARD Nanobody (SAA1187) [SAA1187] (HV826013) is a alpaca monoclonal antibody detecting PYCARD in ELISA, IF, IP. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IF, IP

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clone ID

SAA1187

Clonality

Monoclonal

Target

TMS1, CARD5, Caspase recruitment domain-containing protein 5, Apoptosis-associated speck-like protein containing a CARD, PYD and CARD domain-containing protein, ASC, PYCARD, hASC, Target of methylation-induced silencing 1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column

Accession

Q9ULZ3

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human ASC/TMS1/PYCARD Nanobody (SAA1187)
Immunofluorescence

MCF7 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with His-tagged ASC protein (HV826013) at a concentration of 20 µg/mL overnight at 4°C. After washing, the cells were incubated with an anti-His primary antibody for 1 hour at room temperature, followed by incubation with Goat Anti-Mouse IgG (Alexa Fluor 488) for 50 minutes at room temperature. The localization of IDE was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).