ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
12.14 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
TMS1, CARD5, Caspase recruitment domain-containing protein 5, Apoptosis-associated speck-like protein containing a CARD, PYD and CARD domain-containing protein, ASC, PYCARD, hASC, Target of methylation-induced silencing 1
Background
Apoptosis-associated speck-like protein containing a CARD (ASC/TMS1) is a ~21 kDa protein. Functions as a key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in innate immune response by acting as an integral adapter in the assembly of various inflammasomes (NLRP1, NLRP2, NLRP3, NLRP6, AIM2 and probably IFI16) which recruit and activate caspase-1 leading to processing and secretion of pro-inflammatory cytokines. Caspase-1-dependent inflammation leads to macrophage pyroptosis, a form of cell death.
1. McConnell, BB. et al. (2000) Cancer research 60, 6243-7. PMID: 11103777
2. Masumoto, J. et al. (2003) Biochemical and biophysical research communications 303, 69-73. PMID: 12646168
3. Agostini, L. et al. (2004) Immunity 20, 319-25. PMID: 15030775
4. Faustin, B. et al. (2007) Molecular cell 25, 713-24. PMID: 17349957
5. Fernandes-Alnemri, T. et al. (2007) Cell death and differentiation 14, 1590-604. PMID: 17599095
6. Hornung, V. et al. (2009) Nature 458, 514-8. PMID: 19158675
7. Fernandes-Alnemri, T. et al. (2009) Nature 458, 509-13. PMID: 19158676
8. Bryan, NB. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 3173-82. PMID: 19234215
9. Hasegawa, M. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 7655-62. PMID: 19494289
10. Taxman, DJ. et al. (2011) The Journal of biological chemistry 286, 19605-16. PMID: 21487011
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human ASC/TMS1/PYCARD Protein, N-His
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