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| 카탈로그 번호 | HC491013 | ||||||
|---|---|---|---|---|---|---|---|
| 종 반응성 | Human, Mouse | ||||||
| 응용 분야 | ELISA, FCM, WB | ||||||
| 숙주 종 | Mouse | ||||||
| 클론성 | Monoclonal | ||||||
| 이소타입 | IgG1, kappa | ||||||
| 클론 ID | 6A7 | ||||||
| 타겟 | BAX, Bcl2-L-4, BCL2L4, Bcl-2-like protein 4, Apoptosis regulator BAX | ||||||
| 내독소 수준 | Please contact with the lab for this information. | ||||||
| 순도 | >95% as determined by SDS-PAGE. | ||||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||||
| 등록번호 | Q07812 | ||||||
| RRID | Anti-Human BAX Antibody (6A7) (abinScience Cat# HC491013, RRID:AB_3726396) |
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| 형태 | Liquid | ||||||
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| 참고사항 | For research use only. | ||||||
| Isotype Control | Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207) |
SEC-HPLC detection for Anti-Human BAX Antibody (6A7).
Flow-cytometry using anti-human BAX antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human BAX antibody monoclonal antibody (Catalog # HC491013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human BAX antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human BAX antibody monoclonal antibody (Catalog # HC491013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human BAX antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human BAX antibody monoclonal antibody (Catalog # HC491013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Anti-Human BAX Antibody (6A7).
Flow-cytometry using FITC anti-human BAX antibody. TNFa stimulated U937 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human BAX monoclonal antibody (Catalog: HC491013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using PE anti-human BAX antibody. TNFa stimulated U937 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human BAX monoclonal antibody (Catalog: HC491013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
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