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Anti-Human Coagulation factor III/Tissue Factor Recombinant Antibody (SAA0059) (HB058107)

Anti-Human Coagulation factor III/Tissue Factor Recombinant Antibody (SAA0059)
Anti-Human Coagulation factor III/Tissue Factor Recombinant Antibody (SAA0059)
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개요
카탈로그 번호HB058107
종 반응성Human
응용 분야FCM
숙주 종Human
클론성Monoclonal
이소타입IgG1, kappa
클론 IDSAA0059
테스트된 응용 분야 FCM
타겟 Tissue factor, CD142, F3, Thromboplastin, Coagulation factor III, TF
내독소 수준 Please contact with the lab for this information.
순도 >95% as determined by SDS-PAGE.
정제 Protein A/G purified from cell culture supernatant.
등록번호 P13726
형태 Liquid
저장 완충액 0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
배경

Tissue factor (CD142/F3) is a ~33 kDa protein. Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. CD142 is the therapeutic target of tisotumab vedotin (Tivdak).

참고사항 For research use only.
이미지
  • Anti-Human Coagulation factor III/Tissue Factor Recombinant Antibody (SAA0059)

    Flow Cytometry

    Flow-cytometry using anti-human CD142 antibody.CD142 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD142 antibody monoclonal antibody (Catalog # HB058107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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