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Anti-Human EMMPRIN/CD147 Recombinant Antibody (SAA0061) (HW664107)

Anti-Human EMMPRIN/CD147 Recombinant Antibody (SAA0061)
Anti-Human EMMPRIN/CD147 Recombinant Antibody (SAA0061)
Anti-Human EMMPRIN/CD147 Recombinant Antibody (SAA0061)
Anti-Human EMMPRIN/CD147 Recombinant Antibody (SAA0061)
Anti-Human EMMPRIN/CD147 Recombinant Antibody (SAA0061)
Anti-Human EMMPRIN/CD147 Recombinant Antibody (SAA0061)
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  • 개요

  • 이미지

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  • 데이터시트

  • SDS

개요
카탈로그 번호HW664107
종 반응성Human
응용 분야ELISA, FCM, IHC, WB
숙주 종Human
클론성Monoclonal
이소타입IgG1, kappa
클론 IDSAA0061
테스트된 응용 분야 WB, FCM
타겟 Hepatoma-associated antigen, BSG, HAb18G, 5F7, TCSF, OK blood group antigen, Collagenase stimulatory factor, CD147, Leukocyte activation antigen M6, Basigin, Extracellular matrix metalloproteinase inducer, EMMPRIN, Tumor cell-derived collagenase stimulatory factor
내독소 수준 Please contact with the lab for this information.
순도 >95% as determined by SDS-PAGE.
정제 Protein A/G purified from cell culture supernatant.
등록번호 P35613
형태 Liquid
저장 완충액 0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
배경

Basigin (CD147/BSG) is a ~42 kDa protein. Essential for normal retinal maturation and development. Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors. In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors. May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes.

1. Aït-Ali, N. et al. (2015) Cell 161, 817-32. PMID: 25957687
2. Redzic, JS. et al. (2011) Journal of molecular biology 411, 68-82. PMID: 21620857
참고사항 For research use only.
이미지
  • Anti-Human EMMPRIN/CD147 Recombinant Antibody (SAA0061)

    Bioactivity

    SEC-HPLC detection for Anti-Human CD147/BSG Antibody (ABS1891).

  • Anti-Human EMMPRIN/CD147 Recombinant Antibody (SAA0061)

    Flow Cytometry

    Flow-cytometry using anti-human CD147 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD147 monoclonal antibody (Catalog HW664107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human EMMPRIN/CD147 Recombinant Antibody (SAA0061)

    Western blot

    Western blot analysis was performed using anti-CD147/BSG monoclonal antibody at 1μg/ml on various samples.
    Lane 1: recombinant human CD147/BSG (Catalog No: HW664011)

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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