







| 카탈로그 번호 | HT139107 |
|---|---|
| 종 반응성 | Human |
| 응용 분야 | FCM, blocking |
| 숙주 종 | Mouse |
| 클론성 | Monoclonal |
| 이소타입 | IgG1, kappa |
| 클론 ID | 103.2# |
| 타겟 | BTF5, BTN3A1, Butyrophilin subfamily 3 member A1, CD277 |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >95% as determined by SDS-PAGE. |
| 정제 | Protein A/G purified from cell culture supernatant. |
| 등록번호 | O00481 |
| 형태 | Liquid |
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 대체 이름 | hu103.2 |
| 배경 | Butyrophilin subfamily 3 member A1 (CD277/BTN3A1) is a ~57 kDa protein. Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate. |
| 참고사항 | For research use only. |

Detects CD277/BTN3A1 in indirect ELISAs.

Flow-cytometry using anti-human CD277 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD277 antibody monoclonal antibody (Catalog # HT139107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human CD277/BTN3A1 Antibody (103.2#)

Western blot analysis was performed using anti-CD277/BTN3A1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD277/BTN3A1 (Catalog No: HT139011)
Lane 2: negative control








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