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| 카탈로그 번호 | HY328043 | ||||||
|---|---|---|---|---|---|---|---|
| 종 반응성 | Human | ||||||
| 응용 분야 | ELISA, FCM, WB | ||||||
| 숙주 종 | Human | ||||||
| 클론성 | Monoclonal | ||||||
| 이소타입 | IgG4, kappa | ||||||
| 클론 ID | SAA2009 | ||||||
| 테스트된 응용 분야 | WB, FCM | ||||||
| 타겟 | B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IFNB2, IL-6, IL6, Interferon beta-2, Interleukin-6 | ||||||
| 내독소 수준 | < 10 EU/mg | ||||||
| 순도 | >95% as determined by SDS-PAGE. | ||||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||||
| 등록번호 | P05231 | ||||||
| RRID | Anti-Human IL6 Antibody (SAA2009) (abinScience Cat# HY328043, RRID:AB_3727921) |
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| 형태 | Liquid | ||||||
| 저장 완충액 | 0.01M PBS pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||
| 배경 | Interleukin-6 (IL6) is a ~23 kDa protein. Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable). IL6 is the therapeutic target of tocilizumab (Actemra). | ||||||
| 참고사항 | For research use only. | ||||||
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
SEC-HPLC detection for Anti-Human IL6 Antibody (ABS1221).
Flow-cytometry using anti-human IL6 antibody.IL6 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # HY328043 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Anti-Human IL6 Antibody (SAA2009).
Western blot analysis was performed using anti-IL6 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IL6 (Catalog No: HY328021), 50ng
Lane 2: recombinant human IL6 (Catalog No: HY328021), 30ng
Lane 3: recombinant human IL6 (Catalog No: HY328021), 20ng
Lane 4: recombinant human IL6 (Catalog No: HY328021), 10ng
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