Anti-Human SULT1E1 Polyclonal Antibody (HW729014)

Name: Anti-Human SULT1E1 Polyclonal Antibody
Brand: abinScience
SKU: HW729014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HW729014

설명

Anti-Human SULT1E1 Polyclonal Antibody (HW729014) is a rabbit polyclonal antibody detecting SULT1E1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human SULT1E1 (Met1-Ile294 ).

타겟

ST1E1, STE, EST-1, SULT1E1, Sulfotransferase 1E1, Sulfotransferase, estrogen-preferring, Estrogen sulfotransferase

정제

Purified by antigen affinity column.

등록번호

P49888

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Sulfotransferase 1E1 (SULT1E1) is a ~35 kDa protein. Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. Is a key enzyme in estrogen homeostasis, the sulfation of estrogens leads to their inactivation. Also sulfates dehydroepiandrosterone (DHEA), pregnenolone, (24S)-hydroxycholesterol and xenobiotic compounds like ethinylestradiol, equalenin, diethyl stilbesterol and 1-naphthol at significantly lower efficiency. Does not sulfonate cortisol, testosterone and dopamine. May play a role in gut microbiota-host metabolic interaction.

1. Hempel, N. et al. (2000) Biochemical and biophysical research communications 276, 224-30. PMID: 11006110

2. Pedersen, LC. et al. (2002) The Journal of biological chemistry 277, 17928-32. PMID: 11884392

3. Falany, CN. et al. (1995) The Journal of steroid biochemistry and molecular biology 52, 529-39. PMID: 7779757

4. Cook, IT. et al. (2009) Drug metabolism and disposition: the biological fate of chemicals 37, 2069-78. PMID: 19589875

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-SULT1E1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Mouse brain lysate
Lane 3: Mouse liver lysate
Lane 4: Mouse kidney lysate
Lane 5: Mouse small intestine lysate

참고 문헌

데이터시트

Anti-Human SULT1E1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human SULT1E1 Polyclonal Antibody (HW729014)

개요

이미지

참고 문헌

데이터시트

SDS