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Anti-Human IGF-I/IGF-1 Polyclonal Antibody (HY095014)

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  • 개요

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개요
카탈로그 번호HY095014
종 반응성Human
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human IGF1 (Gly49-Ala118).
타겟 IGF-I, IGF1, Mechano growth factor, MGF, Insulin-like growth factor I, IBP1, Somatomedin-C
정제 Purified by antigen affinity column.
등록번호 P05019
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Insulin-like growth factor 1 (IGF1) is a ~21 kDa protein. The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb.

1. Zoidis, E. et al. (2011) Molecular and cellular biochemistry 348, 33-42. PMID: 21076856
2. Shcheglovitov, A. et al. (2013) Nature 503, 267-71. PMID: 24132240
3. Saegusa, J. et al. (2009) The Journal of biological chemistry 284, 24106-14. PMID: 19578119
4. Fujita, M. et al. (2012) The Journal of biological chemistry 287, 12491-500. PMID: 22351760
5. Fujita, M. et al. (2013) The Journal of biological chemistry 288, 3059-69. PMID: 23243309
6. Fujita, M. et al. (2013) The Journal of biological chemistry 288, 19593-603. PMID: 23696648
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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