Anti-KARS1 Polyclonal Antibody (HC295014)

Name: Anti-KARS1 Polyclonal Antibody
Brand: abinScience
SKU: HC295014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HC295014

설명

Anti-KARS1 Polyclonal Antibody (HC295014) is a rabbit polyclonal antibody detecting KARS1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Cricetulus griseus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human KARS1 (Arg323-Arg553).

타겟

KIAA0070, KARS, Lysyl-tRNA synthetase, LysRS, Lysine--tRNA ligase, KARS1

정제

Purified by antigen affinity column.

등록번호

Q15046

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Lysine--tRNA ligase (KARS1) is a ~68 kDa protein. Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.

1. Guzzo, CM. et al. (2008) Biochemical and biophysical research communications 365, 718-23. PMID: 18029264

2. Guo, M. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 2331-6. PMID: 18272479

3. Shiba, K. et al. (1997) The Journal of biological chemistry 272, 22809-16. PMID: 9278442

4. Park, SG. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 6356-61. PMID: 15851690

5. Lee, YN. et al. (2004) Immunity 20, 145-51. PMID: 14975237

6. Yannay-Cohen, N. et al. (2009) Molecular cell 34, 603-11. PMID: 19524539

7. Ofir-Birin, Y. et al. (2013) Molecular cell 49, 30-42. PMID: 23159739

8. Zamecnik, PC. et al. (1966) Biochemical and biophysical research communications 24, 91-7. PMID: 5338216

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-KARS1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-KARS1 Polyclonal Antibody (HC295014)

개요

이미지

참고 문헌

데이터시트

SDS