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Anti-WT1 Polyclonal Antibody (HB628014)

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개요
카탈로그 번호HB628014
설명
Anti-WT1 Polyclonal Antibody (HB628014) is a rabbit polyclonal antibody detecting WT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Pig, and Xenopus laevis.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human, Mouse, Rat
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human WT1 (Thr317-Gln446).
타겟 WT1, Wilms tumor protein, WT33
정제 Purified by antigen affinity column.
등록번호 P19544
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Wilms tumor protein (WT1) is a ~49 kDa protein. Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation.

1. Hamilton, TB. et al. (1995) Nucleic acids research 23, 277-84. PMID: 7862533
2. Stoll, R. et al. (2007) Journal of molecular biology 372, 1227-45. PMID: 17716689
3. Hashimoto, H. et al. (2014) Genes & development 28, 2304-13. PMID: 25258363
4. Smolen, GA. et al. (2004) Cancer research 64, 7846-51. PMID: 15520190
5. Markus, MA. et al. (2006) Experimental cell research 312, 3379-88. PMID: 16934801
6. Weiss, TC. et al. (2009) Biochemistry 48, 148-55. PMID: 19123921
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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