HEK293T Human CD142/F3/TF Stable Cell Line (HB058818)

카탈로그 번호	HB058818
설명	HEK293T Human CD142/F3/TF Stable Cell Line (HB058818) is a stable cell line expressing CD142/F3/TF. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
등록번호	P13726
응용 분야	FCM
숙주 종	HEK293T
종	Human
타겟	CD142/F3/TF
특성	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
안정성 및 저장	Frozen in liquid nitrogen.
사양	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
배경	Tissue factor (CD142/F3) is a ~33 kDa protein. Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. CD142 is the therapeutic target of tisotumab vedotin (Tivdak).

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD142 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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