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InVivoMAb Anti-Human IL-6 Antibody (Iv0022) (HY328010)

InVivoMAb Anti-Human IL-6 Antibody (Iv0022)
InVivoMAb Anti-Human IL-6 Antibody (Iv0022)
InVivoMAb Anti-Human IL-6 Antibody (Iv0022)
InVivoMAb Anti-Human IL-6 Antibody (Iv0022)
InVivoMAb Anti-Human IL-6 Antibody (Iv0022)
InVivoMAb Anti-Human IL-6 Antibody (Iv0022)
InVivoMAb Anti-Human IL-6 Antibody (Iv0022)
InVivoMAb Anti-Human IL-6 Antibody (Iv0022)
InVivoMAb Anti-Human IL-6 Antibody (Iv0022)
InVivoMAb Anti-Human IL-6 Antibody (Iv0022)
가격(USD):
사양:
  • 100ug
  • 1mg
수량:
문의하기
  • 개요

  • 이미지

  • 참고 문헌

  • 데이터시트

  • SDS

개요
카탈로그 번호HY328010
설명
InVivoMAb Anti-Human IL6 (Iv0022) [Iv0022] (HY328010) is a human monoclonal antibody detecting Hybridoma growth factor in ELISA, FCM, Neutralization. Suitable for Human.
Highlights
  • ●Multi-Application — Validated across multiple applications.
  • ●Flow Cytometry — Validated for flow cytometry applications.
종 반응성Human
응용 분야ELISA, FCM, WB, Neutralization
숙주 종Human
클론성Monoclonal
이소타입IgG1, kappa
클론 IDIv0022
테스트된 응용 분야 WB, FCM
타겟 B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IFNB2, IL-6, IL6, Interferon beta-2, Interleukin-6
내독소 수준 < 1 EU/mg
순도 >95% as determined by SDS-PAGE.
관련 제품 pH 7.4 Dilution Buffer
정제 Protein A/G purified from cell culture supernatant.
등록번호 P05231
형태 Liquid
저장 완충액 0.01M PBS pH 7.4.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:2000-1:20000
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
참고사항 For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
이미지
  • InVivoMAb Anti-Human IL-6 Antibody (Iv0022)

    Bioactivity

    SEC-HPLC detection for InVivoMAb Anti-Human IL6 (ABV0244).

  • InVivoMAb Anti-Human IL-6 Antibody (Iv0022)

    Flow Cytometry

    Flow-cytometry using anti-human IL6 antibody.IL6 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # HY328010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • InVivoMAb Anti-Human IL-6 Antibody (Iv0022)

    Flow Cytometry

    Flow-cytometry using anti-human IL6 antibody.LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # HY328010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • InVivoMAb Anti-Human IL-6 Antibody (Iv0022)

    SDS-PAGE

    SDS-PAGE for InVivoMAb Anti-Human IL6 (Iv0022).

  • InVivoMAb Anti-Human IL-6 Antibody (Iv0022)

    Western blot

    Western blot analysis was performed using anti-IL6 monoclonal antibody at 1μg/ml on various samples.
    Lane 1: recombinant human IL6 (Catalog No: HY328021), 50ng
    Lane 2: recombinant human IL6 (Catalog No: HY328021), 30ng
    Lane 3: recombinant human IL6 (Catalog No: HY328021), 20ng
    Lane 4: recombinant human IL6 (Catalog No: HY328021), 10ng

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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