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| 카탈로그 번호 | HW398012 |
|---|---|
| 설명 |
Recombinant Human CD130/IL6ST Protein, N-His (HW398012) expressed in E. coli, spanning Thr392-Thr566. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P40189 |
| 단백질 길이 | Thr392-Thr566 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 22.05 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | IL-6 receptor subunit beta, CDw130, IL-6R-beta, Membrane glycoprotein 130, CD130, Oncostatin-M receptor subunit alpha, Interleukin-6 signal transducer, IL-6RB, IL-6R subunit beta, IL6ST, Interleukin-6 receptor subunit beta, gp130 |
| 배경 | Interleukin-6 receptor subunit beta (CD130/IL6ST) is a ~103 kDa protein. Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates the intracellular JAK-MAPK and JAK-STAT3 signaling pathways. That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3. In parallel, the IL6 signaling pathway induces the expression of two cytokine receptor signaling inhibitors, SOCS1 and SOCS3, which inhibit JAK and terminate the activity of the IL6 signaling pathway as a negative feedback loop. 1. Hibi, M. et al. (1990) Cell 63, 1149-57. PMID: 2261637 2. Waetzig, GH. et al. (2010) The Journal of biological chemistry 285, 1781-9. PMID: 19915009 3. Schütt, A. et al. (2013) The Biochemical journal 450, 487-96. PMID: 23294003 4. Taniguchi, K. et al. (2015) Nature 519, 57-62. PMID: 25731159 5. Hashimoto, Y. et al. (2009) Molecular biology of the cell 20, 2864-73. PMID: 19386761 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human CD130/IL6ST Protein, N-His
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