Anti-CD130/IL6ST Polyclonal Antibody (HW398014)

Name: Anti-CD130/IL6ST Polyclonal Antibody
Brand: abinScience
SKU: HW398014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW398014

Description

Anti-CD130/IL6ST Polyclonal Antibody (HW398014) is a rabbit polyclonal antibody detecting CD130 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD130/IL6ST (Thr392-Thr566).

Target

IL-6 receptor subunit beta, CDw130, IL-6R-beta, Membrane glycoprotein 130, CD130, Oncostatin-M receptor subunit alpha, Interleukin-6 signal transducer, IL-6RB, IL-6R subunit beta, IL6ST, Interleukin-6 receptor subunit beta, gp130

Purification

Purified by antigen affinity column.

Accession

P40189

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interleukin-6 receptor subunit beta (CD130/IL6ST) is a ~103 kDa protein. Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates the intracellular JAK-MAPK and JAK-STAT3 signaling pathways. That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3. In parallel, the IL6 signaling pathway induces the expression of two cytokine receptor signaling inhibitors, SOCS1 and SOCS3, which inhibit JAK and terminate the activity of the IL6 signaling pathway as a negative feedback loop.

1. Hibi, M. et al. (1990) Cell 63, 1149-57. PMID: 2261637

2. Waetzig, GH. et al. (2010) The Journal of biological chemistry 285, 1781-9. PMID: 19915009

3. Schütt, A. et al. (2013) The Biochemical journal 450, 487-96. PMID: 23294003

4. Taniguchi, K. et al. (2015) Nature 519, 57-62. PMID: 25731159

5. Hashimoto, Y. et al. (2009) Molecular biology of the cell 20, 2864-73. PMID: 19386761

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD130/IL6ST polyclonal antibody at 1ug/mL on recombinant CD130/IL6ST (Catalog No. HW398012).

References

Datasheet