Human ICAM-1/CD54 Recombinant Protein (N-His) (HY321012)

Name: Human ICAM-1/CD54 Recombinant Protein (N-His)
Brand: abinScience
SKU: HY321012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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개요
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참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HY321012
설명	Recombinant Human CD54/ICAM1 Protein, N-His (HY321012) expressed in E. coli, spanning Gln28-Glu480. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	E. coli
등록번호	P05362
단백질 길이	Gln28-Glu480
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
예측 분자량	51.8 kDa
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	ICAM1, Major group rhinovirus receptor, CD54, ICAM-1, Intercellular adhesion molecule 1
배경	Intercellular adhesion molecule 1 (CD54/ICAM1) is a ~57 kDa protein. Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL:ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring leukocyte cell-cell adhesion, by providing a calibrated system to namely adjust T-cell killing to the antigen stimulation strength. Also functions as a ligand receptor of the signaling receptor ITGAM:ITGB2/MAC-1 ensuring adhesion between stimulated neutrophils and stimulated endothelial cells. During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. Promotes cell aggregation in epithelial cells through interaction with MUC1. 1. Dustin, ML. et al. (1986) Journal of immunology (Baltimore, Md. : 1950) 137, 245-54. PMID: 3086451 2. Simmons, D. et al. (1988) Nature 331, 624-7. PMID: 3340213 3. Lacouture, C. et al. (2024) Nature communications 15, 407. PMID: 38195629 4. Diamond, MS. et al. (1990) The Journal of cell biology 111, 3129-39. PMID: 1980124 5. van Buul, JD. et al. (2007) The Journal of cell biology 178, 1279-93. PMID: 17875742 6. Hayashi, T. et al. (2001) Digestion 63 Suppl 1, 87-92. PMID: 11173916
참고사항	For research use only.