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Human CEACAM6/CD66c Recombinant Protein (N-His) (HW494012)

Human CEACAM6/CD66c Recombinant Protein (N-His)
Human CEACAM6/CD66c Recombinant Protein (N-His)
Human CEACAM6/CD66c Recombinant Protein (N-His)
Human CEACAM6/CD66c Recombinant Protein (N-His)
가격(USD):
사양:
  • 50ug
  • 100ug
  • 1mg
수량:
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  • 개요

  • 이미지

  • 참고 문헌

  • 데이터시트

  • SDS

개요
카탈로그 번호HW494012
설명
Recombinant Human CD66c/CEACAM6 Protein, N-His (HW494012) expressed in E. coli, spanning Lys35-Gly320. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템E. coli
등록번호P40199
단백질 길이Lys35-Gly320
응용 분야ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종Homo sapiens (Human)
성질 Recombinant
테스트된 응용 분야 WB
내독소 수준 Please contact with the lab for this information.
순도 >90% as determined by SDS-PAGE.
예측 분자량 33.55 kDa
형태 Lyophilized
저장 완충액 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

재구성 Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
배송 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름Cell adhesion molecule CEACAM6, Carcinoembryonic antigen-related cell adhesion molecule 6, CEA cell adhesion molecule 6, Non-specific crossreacting antigen, Normal cross-reacting antigen, CD66c, CEACAM6, NCA
배경

Cell adhesion molecule CEACAM6 is a ~37 kDa protein. Cell surface glycoprotein that plays a role in cell adhesion and tumor progression. Intercellular adhesion occurs in a calcium- and fibronectin-independent manner. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8. Heterophilic interaction with CEACAM8 occurs in activated neutrophils. Plays a role in neutrophil adhesion to cytokine-activated endothelial cells.

1. Ordoñez, C. et al. (2000) Cancer research 60, 3419-24. PMID: 10910050
2. Kuroki, M. et al. (2001) Journal of leukocyte biology 70, 543-50. PMID: 11590190
3. Kuijpers, TW. et al. (1992) The Journal of cell biology 118, 457-66. PMID: 1378450
4. Blumenthal, RD. et al. (2005) Cancer research 65, 8809-17. PMID: 16204051
5. Oikawa, S. et al. (1991) The Journal of biological chemistry 266, 7995-8001. PMID: 2022629
6. Oikawa, S. et al. (1989) Biochemical and biophysical research communications 164, 39-45. PMID: 2803308
7. Yamanaka, T. et al. (1996) Protein expression and purification 7, 438-46. PMID: 8776764
참고사항 For research use only
이미지
  • Human CEACAM6/CD66c Recombinant Protein (N-His)

    Western blot

    Detected by Anti-CD66c/CEACAM6 monoclonal antibody (Catalog No: HW494046)

  • Human CEACAM6/CD66c Recombinant Protein (N-His)

    Bioactivity

    Detected by Research Grade Anti-Human CD66c/CEACAM6 (NOV1803) (Catalog No: HW494046) in indirect ELISA

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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