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| 카탈로그 번호 | HP137012 |
|---|---|
| 설명 |
Recombinant Human DZIP1 Protein, N-His-SUMO (HP137012) expressed in E. coli, spanning Ser66-Asp114. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q86YF9 |
| 단백질 길이 | Ser66-Asp114 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 17.80 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | DAZ-interacting protein 1/2, DZIP1, Zinc finger protein DZIP1, DZIP, KIAA0996, DZIP2 |
| 배경 | Cilium assembly protein DZIP1 is a ~98 kDa protein. Molecular adapter that recruits protein complexes required for cilium assembly and function to the cilium basal body. At the exit of mitosis, localizes to the basal body and ciliary base of the forming primary cilium where it recruits and activates RAB8A to direct vesicle-mediated transport of proteins to the cilium. Also recruits the BBSome, a complex involved in cilium biogenesis, by bridging it to PCM1 at the centriolar satellites of the cilium. It is also required for the recruitment to the cilium basal body of the intraflagellar transport (IFT) machinery as well as the ciliary appendage proteins CEP164 and NINEIN. Functions as a regulator of Hedgehog signaling both through its role in cilium assembly but also probably through its ability to retain GLI3 within the cytoplasm. 1. Glazer, AM. et al. (2010) Developmental biology 337, 148-56. PMID: 19852954 2. Wang, C. et al. (2013) The Journal of biological chemistry 288, 29518-29. PMID: 23955340 3. Zhang, B. et al. (2017) The Journal of biological chemistry 292, 1351-1360. PMID: 27979967 4. Lv, M. et al. (2020) Journal of medical genetics 57, 445-453. PMID: 32051257 5. Toomer, KA. et al. (2019) Science translational medicine 11. PMID: 31118289 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human DZIP1 Protein, N-His-SUMO
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