Anti-Human ICAM-3/CD50 Reference Antibody (ICM3, RUO) (HW443013)

Name: Anti-Human ICAM-3/CD50 Reference Antibody (ICM3, RUO)
Brand: abinScience
SKU: HW443013
Price: 176 USD
Availability: InStock

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사양:

100ug
1mg
5mg
10mg
25mg
50mg
100mg

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HW443013

설명

Anti-Human CD50/ICAM3 (ICM3) (HW443013) is a research-grade recombinant antibody targeting CD50. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

종 반응성

Human

응용 분야

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

숙주 종

Human

클론성

Monoclonal

이소타입

IgG1

클론 ID

ICM3

발현 시스템

Mammalian cells

타겟

CD50, CDw50, Intercellular adhesion molecule 3, ICAM-R, ICAM3, ICAM-3

내독소 수준

< 10 EU/mg

순도

>95% purity as determined by SDS-PAGE.

정제

Protein A/G purified from cell culture supernatant.

등록번호

P32942

형태

Liquid

저장 완충액

0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:2000-1:20000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Intercellular adhesion molecule 3 (CD50/ICAM3) is a ~59 kDa protein. Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL:ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring apoptotic neutrophil phagocytosis by macrophages. Is also a ligand for integrin ITGAD/ITGB2. Ligand of CD209 on dendritic cells (DC) through an integrin-independent mechanism that requires Ca(2+) and that forms a first contact between DC and resting T cell, facilitating the downstream DC-T cell clustering process and DC-induced proliferation of resting T Cells.

1. Fawcett, J. et al. (1992) Nature 360, 481-4. PMID: 1448173

2. Vazeux, R. et al. (1992) Nature 360, 485-8. PMID: 1448174

3. Kristóf, E. et al. (2013) Apoptosis : an international journal on programmed cell death 18, 1235-51. PMID: 23775590

4. Juan, M. et al. (1993) European journal of immunology 23, 1508-12. PMID: 8325327

5. de Fougerolles, AR. et al. (1993) The Journal of experimental medicine 177, 1187-92. PMID: 8459213

6. Van der Vieren, M. et al. (1995) Immunity 3, 683-90. PMID: 8777714

7. Geijtenbeek, TB. et al. (2000) Cell 100, 575-85. PMID: 10721994

참고사항

For research use only. Not suitable for clinical or therapeutic use.

Isotype Control

Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

이미지

Bioactivity

Detects Human CD50/ICAM3 in indirect ELISAs.
Bioactivity

SEC-HPLC detection for Research Grade Anti-Human CD50/ICAM3 (ICM3).
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD50/ICAM3 (ICM3)
Flow CytoMetry

Flow-cytometry using APC anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human CD50 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.