Anti-Human ICAM-3/CD50 Recombinant Antibody (SAA1382) (HW443107)

Name: Anti-Human ICAM-3/CD50 Recombinant Antibody (SAA1382)
Brand: abinScience
SKU: HW443107
Price: 196 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	HW443107
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1
Clone ID	SAA1382
Clonality	Monoclonal
Target	CD50, CDw50, Intercellular adhesion molecule 3, ICAM-R, ICAM3, ICAM-3
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P32942
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Intercellular adhesion molecule 3 (CD50/ICAM3) is a ~59 kDa protein. Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL:ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring apoptotic neutrophil phagocytosis by macrophages. Is also a ligand for integrin ITGAD/ITGB2. Ligand of CD209 on dendritic cells (DC) through an integrin-independent mechanism that requires Ca(2+) and that forms a first contact between DC and resting T cell, facilitating the downstream DC-T cell clustering process and DC-induced proliferation of resting T Cells. 1. Fawcett, J. et al. (1992) Nature 360, 481-4. PMID: 1448173 2. Vazeux, R. et al. (1992) Nature 360, 485-8. PMID: 1448174 3. Kristóf, E. et al. (2013) Apoptosis : an international journal on programmed cell death 18, 1235-51. PMID: 23775590 4. Juan, M. et al. (1993) European journal of immunology 23, 1508-12. PMID: 8325327 5. de Fougerolles, AR. et al. (1993) The Journal of experimental medicine 177, 1187-92. PMID: 8459213 6. Van der Vieren, M. et al. (1995) Immunity 3, 683-90. PMID: 8777714 7. Geijtenbeek, TB. et al. (2000) Cell 100, 575-85. PMID: 10721994
Note	For research use only.

Images

Bioactivity

Detects Human CD50/ICAM3 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD50 antibody.RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD50 antibody monoclonal antibody (Catalog # HW443107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD50 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD50 antibody monoclonal antibody (Catalog # HW443107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.