Anti-Human CD50/ICAM3 Recombinant Antibody (SAA1382), APC (HW443137)

Name: Anti-Human CD50/ICAM3 Recombinant Antibody (SAA1382), APC
Brand: abinScience
SKU: HW443137
Price: 256 USD
Availability: InStock

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50T
100T

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	HW443137
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1
Clone ID	SAA1382
Conjugation	APC
Clonality	Monoclonal
Target	CD50, CDw50, Intercellular adhesion molecule 3, ICAM-R, ICAM3, ICAM-3
Endotoxin level	Please contact with the lab for this information.
Associated products	Mouse IgG1 Recombinant Isotype Control Antibody (HyHEL-10), APC
Accession	P32942
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Intercellular adhesion molecule 3 (CD50/ICAM3) is a ~59 kDa protein. Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL:ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring apoptotic neutrophil phagocytosis by macrophages. Is also a ligand for integrin ITGAD/ITGB2. Ligand of CD209 on dendritic cells (DC) through an integrin-independent mechanism that requires Ca(2+) and that forms a first contact between DC and resting T cell, facilitating the downstream DC-T cell clustering process and DC-induced proliferation of resting T Cells. 1. Fawcett, J. et al. (1992) Nature 360, 481-4. PMID: 1448173 2. Vazeux, R. et al. (1992) Nature 360, 485-8. PMID: 1448174 3. Kristóf, E. et al. (2013) Apoptosis : an international journal on programmed cell death 18, 1235-51. PMID: 23775590 4. Juan, M. et al. (1993) European journal of immunology 23, 1508-12. PMID: 8325327 5. de Fougerolles, AR. et al. (1993) The Journal of experimental medicine 177, 1187-92. PMID: 8459213 6. Van der Vieren, M. et al. (1995) Immunity 3, 683-90. PMID: 8777714 7. Geijtenbeek, TB. et al. (2000) Cell 100, 575-85. PMID: 10721994
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow CytoMetry

Flow-cytometry using APC anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD50 monoclonal antibody (Catalog: HW443137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet