Anti-Human ICAM-3/CD50 Reference Antibody (ICM3, RUO) (HW443013)

Name: Anti-Human ICAM-3/CD50 Reference Antibody (ICM3, RUO)
Brand: abinScience
SKU: HW443013
Price: 176 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HW443013

Description

Anti-Human CD50/ICAM3 (ICM3) (HW443013) is a research-grade recombinant antibody targeting CD50. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

Species reactivity

Human

Applications

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

Host species

Human

Isotype

IgG1

Clone ID

ICM3

Expression system

Mammalian cells

Clonality

Monoclonal

Target

CD50, CDw50, Intercellular adhesion molecule 3, ICAM-R, ICAM3, ICAM-3

Endotoxin level

Please contact the lab for this information.

Purity

>95% purity as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P32942

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Intercellular adhesion molecule 3 (CD50/ICAM3) is a ~59 kDa protein. Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL:ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring apoptotic neutrophil phagocytosis by macrophages. Is also a ligand for integrin ITGAD/ITGB2. Ligand of CD209 on dendritic cells (DC) through an integrin-independent mechanism that requires Ca(2+) and that forms a first contact between DC and resting T cell, facilitating the downstream DC-T cell clustering process and DC-induced proliferation of resting T Cells.

1. Fawcett, J. et al. (1992) Nature 360, 481-4. PMID: 1448173

2. Vazeux, R. et al. (1992) Nature 360, 485-8. PMID: 1448174

3. Kristóf, E. et al. (2013) Apoptosis : an international journal on programmed cell death 18, 1235-51. PMID: 23775590

4. Juan, M. et al. (1993) European journal of immunology 23, 1508-12. PMID: 8325327

5. de Fougerolles, AR. et al. (1993) The Journal of experimental medicine 177, 1187-92. PMID: 8459213

6. Van der Vieren, M. et al. (1995) Immunity 3, 683-90. PMID: 8777714

7. Geijtenbeek, TB. et al. (2000) Cell 100, 575-85. PMID: 10721994

Note

For research use only. Not suitable for clinical or therapeutic use.

Images

Bioactivity

Detects Human CD50/ICAM3 in indirect ELISAs.
Bioactivity

SEC-HPLC detection for Research Grade Anti-Human CD50/ICAM3 (ICM3).
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD50/ICAM3 (ICM3)
Flow CytoMetry

Flow-cytometry using APC anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human CD50 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.