Anti-Human Coagulation factor III/Tissue Factor Reference Antibody (Tisotumab, RUO) (HB058016)

Name: Anti-Human Coagulation factor III/Tissue Factor Reference Antibody (Tisotumab, RUO)
Brand: abinScience
SKU: HB058016
Price: 176 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HB058016
설명	Tisotumab (HB058016) is a research-grade recombinant antibody targeting CD142. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
종 반응성	Human
응용 분야	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
숙주 종	Human
클론성	Monoclonal
이소타입	IgG1-kappa
발현 시스템	Mammalian cells
테스트된 응용 분야	WB, FCM
타겟	Tissue factor, CD142, F3, Thromboplastin, Coagulation factor III, TF
내독소 수준	< 10 EU/mg
순도	>95% purity as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	P13726
형태	Liquid
저장 완충액	0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	HuMax-TF-ADC, TF-011-MMAE, HuMax-TF, 1418628-81-5
배경	Tissue factor (CD142/F3) is a ~33 kDa protein. Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. CD142 is the therapeutic target of tisotumab vedotin (Tivdak).
참고사항	For research use only. Not suitable for clinical or therapeutic use.

이미지

SEC-HPLC

SEC-HPLC detection for Research Grade Tisotumab.
Bioactivity

Detects CD142/F3/TF in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Tisotumab
Flow CytoMetry

Flow-cytometry using anti-human CD142 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD142 monoclonal antibody (Catalog: HB058016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD142/F3/TF monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD142/F3/TF (Catalog No: HB058011), 50ng
Lane 2: recombinant human CD142/F3/TF (Catalog No: HB058011), 30ng
Lane 3: recombinant human CD142/F3/TF (Catalog No: HB058011), 20ng
Lane 4: recombinant human CD142/F3/TF (Catalog No: HB058011), 10ng
Flow cytometry

Flow-cytometry using APC anti-human CD142 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD142 monoclonal antibody (Catalog HB058016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using PE anti-human CD142 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD142 monoclonal antibody (Catalog HB058016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using FITC anti-human CD142 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD142 monoclonal antibody (Catalog HB058016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.